MSD in form of Primary Structure can be transformed into Genetic inforamtion simply using ORFFinder

Transforming of MSD.Tertiary Structure based Primary structure to Genetic Information for Enterovirus PROBE Design and Engineering Major Structural Differences(MSD) in the form of tertiary Structure based Primary Structure can be transformed into Genetic inforamtion simply by using ORFFinder Application

ORFFinder https://www.ncbi.nlm.nih.gov/orffinder/

Set EV71 as Structural Detector

RMSD.EV71 versus CVA6 = 0.89 Aungstrom

RMSD.EV71 versus CVA10 = 0.55 Aungstrom

RMSD.EV71 versus CVA16 = 0.50 Aungstrom

RMSD.EV71 versus EVD68 = 0.73 Aungstrom

RMSD.EV71 versus EVD70 = 0.74 Aungstrom

RMSD.EV71 versus CVB1 = 0.71 Aungstrom

RMSD.EV71 versus E1 = 0.79 Aungstrom

RMSD.EV71 versus CVA1 = 0.78 Aungstrom

RMSD.EV71 versus PV1 = 0.78 Aungstrom

Targeted MSD.Enterovirus Genome.VP2

>EV71_VP2_MSD-AA129-157 EYVIGTVAGGTGTENSHPPYKQTQPGADG

>CVA6_VP2_MSD-AA129-157 EFVVAASSPATKPNGQGLYPDFAHTNPGK

>CVA10_VP2_MSD-AA129-156 EFVLAGRGSNTKPNEAPHPGFNTTFPGT

>CVA16_VP2_MSD-AA129-156 EYVLGTIAGGDGNENSHPPYVTTQPGQV

>CVB1_AA128-150 PEAEMGCSNLNNTPKFAELSGGD

>E1_AA128-150 PEAEMGSAQTSGVVNYEHISKGE

>CVA1_AA129-152 EFMMGSNTDTNTGGITYEKANPGE

>PV1_AA129-152 EMCLAGDSNTTTMHTSYQNANPGE

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>EV71_VP2_MSD2-AA218-239 PISPLDFDQGATPVIPITITLA

>EVD68_VP2_MSD2-AA206-238 QWTLAVIPVVPLGTRTMSSVVPITVSIAPMCCE

>EVD70_VP2_MSD2-AA208-240 TLAVIPVCPLAGGTGNTNTYVPITISIAPMCAE

FINAL.Transforming of MSD.Tertiary Structure based Primary

>EV71_VP2_MSD-AA129-157 E Y V I G T V A G G T G T E N S H P P Y K Q T Q P G A D G GAGTATGTCATTGGAACAGTGGCAGGCGGCACAGGCACAGAGAACAGTCACCCTCCTTATAAACAAACCCAACCCGGCGCTGATGGA

hEV A71 BrCr-USA-70 100% 9e-40 100% JN874547.1

>CVA6_VP2_MSD-AA129-157 E F V V A A S S P A T K P N G Q G L Y P D F A H T N P G K GAGTTCGTGGTTGCTGCGAGCAGCCCTGCTACGAAGCCCAACGGACAAGGGTTGTACCCTGACTTTGCTCACACCAACCCGGGCAAA

Human CV A6 strain Gdula, complete genome 100% 1e-39 100% AY421764.1

>CVA10_VP2_MSD-AA129-156 E F V L A G R G S N T K P N E A P H P G F N T T F P G T GAGTTCGTTCTAGCAGGCAGAGGTTCAAACACGAAGCCCAATGAAGCCCCTCACCCGGGATTTAATACCACCTTTCCTGGCACT Human CV A10 strain Kowalik, complete genome 100% 5e-38 100% AY421767.1

>CVA16_VP2_MSD-AA129-156 E Y V L G T I A G G D G N E N S H P P Y V T T Q P G Q V GAGTACGTGCTGGGCACCATTGCCGGGGGCGATGGTAACGAGAACTCACATCCCCCGTACGTCACCACCCAGCCAGGACAGGTG Human CV A16 complete genome 100% 4e-38 100% EU812514.1

>CVB1_AA128-150 P E A E M G C S N L N N T P K F A E L S G G D CCGGAAGCGGAAATGGGATGCTCAAATCTAAACAACACCCCAAAGTTTGCTGAACTTTCCGGAGGAGAT

Human CV B1 polyprotein gene, complete cds 100% 8e-30 100% EU147493.1

>E1_AA128-150 P E A E M G S A Q T S G V V N Y E H I S K G E CCTGAGGCGGAGATGGGAAGTGCCCAAACCTCAGGGGTGGTCAACTACGAACACATTAGTAAGGGTGAG

Echovirus 1 strain Farouk/ATCC VR-1038 polyprotein 100% 7e-30 100% AF029859.2

>CVA1_AA129-152 E F M M G S N T D T N T G G I T Y E K A N P G E GAGTTTATGATGGGGTCCAACACAGATACTAATACAGGGGGCATAACTTATGAAAAGGCTAATCCTGGTGAA

Human coxsackievirus A1 strain Tompkins, complete genome 100% 2e-31 100% AF499635.1

>PV1_AA129-152 E M C L A G D S N T T T M H T S Y Q N A N P G E GAGATGTGTCTGGCCGGGGATAGCAACACCACTACCATGCACACCAGCTATCAAAATGCCAATCCTGGCGAG

Human poliovirus 1 isolate 18339, complete genome 100% 2e-31 100% MG212479.1

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>EV71_VP2_MSD2-AA218-239 P I S P L D F D Q G A T P V I P I T I T L A CCTATCAGCCCGCTGGATTTCGACCAAGGGGCGACACCGGTAATTCCTATCACTATCACGTTGGCT

Human enterovirus 71 genomic RNA, complete genome, sub_strain:BrCr-ts 100% 4e-28 100% AB204853.1

Human enterovirus 71 genomic RNA, complete genome, sub_strain:BrCr-TR 100% 4e-28 100% AB204852.1

>EVD68_VP2_MSD2-AA206-238 Q W T L A V I P V V P L G T R T M S S V V P I T V S I A P M C C E CAGTGGACACTAGCAGTAATACCAGTGGTTCCATTGGGTACACGCACAATGTCAAGTGTTGTCCCAATAACAGTTTCAATTGCCCCTATGTGTTGTGAG

Enterovirus D68 isolate US/CA/Fermon polyprotein gene, partial cds 100% 3e-46 100% KU844179.1

Enterovirus D68 isolate US/CA/Rhyne polyprotein gene, complete cds 100% 3e-46 100% KU844178.1

>EVD70_VP2_MSD2-AA208-240 T L A V I P V C P L A G G T G N T N T Y V P I T I S I A P M C A E ACATTAGCCGTTATTCCGGTATGTCCATTGGCAGGAGGTACTGGCAACACAAACACCTATGTGCCAATTACCATCTCCATTGCTCCCATGTGTGCTGAA

Human enterovirus 70 strain J670/71, complete genome 100% 3e-46 100% DQ201177.1

Human enterovirus 70 genomic RNA, complete genome, strain: J670/71 100% 3e-46 100% D00820.1