Tertiary Structure based Major Structural Difference can be transformed into Primary Structure

Major Structural Difference in the form of Tertiary Structure(in the figure) can be transformed into Primary Structure.

EV71 VP2 Homology Model was set as Structural Detector where

RMSD.EV71 versus CVA6 = 0.89 Aungstrom

RMSD.EV71 versus CVA10 = 0.55 Aungstrom

RMSD.EV71 versus CVA16 = 0.50 Aungstrom

Detected Major Structural Difference in the form of Tertiary Structure can be transformed into Primary Structure and demonstrated below;

>EV71_VP2_MSD-AA129-157

EYVIGTVAGGTGTENSHPPYKQTQPGADG

capsid protein VP2 [Enterovirus A] 100% 6e-24 100% AFG16966.1

polyprotein [Enterovirus A71] 100% 1e-23 100% AVQ54963.1

polyprotein [Enterovirus A71] 100% 1e-23 100% AJQ21566.1

polyprotein [Enterovirus A71] 100% 1e-23 100% AFN20470.1

>CVA6_VP2_MSD-AA129-157

EFVVAASSPATKPNGQGLYPDFAHTNPGK

VP2 [Coxsackievirus A6] 100% 5e-24 100% AFM77320.1

VP2 [Coxsackievirus A6] 100% 6e-24 100% AFM77318.1

capsid protein [Enterovirus A] 100% 8e-24 100% AGI95626.1

capsid protein [Enterovirus A] 100% 8e-24 100% AGI95625.1

>CVA10_VP2_MSD-AA129-156

EFVLAGRGSNTKPNEAPHPGFNTTFPGT

polyprotein [Coxsackievirus A10] 100% 4e-23 100% AAR38847.1

VP2 [Coxsackievirus A10] 100% 1e-20 93% AFM77334.1

>CVA16_VP2_MSD-AA129-156

EYVLGTIAGGDGNENSHPPYVTTQPGQV

polyprotein [Coxsackievirus A16] 100% 3e-23 100% ACF21979.1

genome polyprotein [Enterovirus A] 100% 3e-23 100% NP_042242.1

VP2 (1B) [Enterovirus A] 100% 3e-23 100% NP_740527.1

The next question is how does it be transformed into genetic information for to be used for PROBE Design and ENGINEERING ?

>EV71_VP2_MSD-AA129-157

EYVIGTVAGGTGTENSHPPYKQTQPGADG

MSD.Genetic Information

GAGTATGTCATTGGAACAGTGGCAGGCGGCACAGGCACAGAGAACAGTCACCCTCCTTATAAACAAACCCAACCCGGCGCTGATGGA

hEVA71 strain A-BrCr-USA-70 polyprotein gene, partial cds 100% 1e-39 100% JN874547.1

hEVA71 polyprotein gene, complete cds 100% 1e-39 100% U22521.1

hEVA71 isolate EV71/wuhan/3018/2010, complete genome 100% 5e-38 99% KF501389.1

hEVA71 polyprotein, VP4, VP2, VP3, VP1 BrCr 100% 5e-38 99% AB777928.1

hEVA71 strain EV71-Hubei-09-China, complete genome 100% 5e-38 99% GU434678.1

hEVA71 genomic RNA, complete genome, sub_strain:BrCr-ts 100% 5e-38 99% AB204853.1

hEVA71 genomic RNA, complete genome, sub_strain:BrCr-TR 100% 5e-38 99% AB204852.1

hEVA71 strain HCM84-VNM-2011, complete genome 98% 4e-24 90% KC296444.1

hEVA71 strain E2005125-TW polyprotein gene, complete cds 98% 4e-24 90% EF063152.1

hEVA71 strain 306A, complete genome 98% 4e-19 86% KJ746493.1

hEVA71 strain SZ/HK08-6, complete genome 98% 2e-17 85% GQ279370.1

hEVA71 strain 4/SHENZHEN/08/China/HFMD/2008, complete genome 98% 2e-17 85% FJ607335.1

hEVA71 strain 1/SHENZHEN/08/China/HFMD/2008, complete genome 98% 2e-17 85% FJ607334.1

hEVA71 isolate EV71/GD10-45/2010, complete genome 98% 8e-16 84% KJ004560.1

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>CVA6_VP2_MSD-AA129-157

EFVVAASSPATKPNGQGLYPDFAHTNPGK

MSD.Genetic Information

GAGTTCGTGGTTGCTGCGAGCAGCCCTGCTACGAAGCCCAACGGACAAGGGTTGTACCCTGACTTTGCTCACACCAACCCGGGCAAA

Human coxsackievirus A6 strain Gdula, complete genome 100% 1e-39 100% AY421764.1

Coxsackievirus A6 strain SHAPHC5644/SH/CHN/14, complete genome 100% 5e-23 89% KX064303.1

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>CVA10_VP2_MSD-AA129-156

EFVLAGRGSNTKPNEAPHPGFNTTFPGT

MSD.Genetic Information

GAGTTCGTTCTAGCAGGCAGAGGTTCAAACACGAAGCCCAATGAAGCCCCTCACCCGGGATTTAATACCACCTTTCCTGGCACT

Human coxsackievirus A10 strain Kowalik, complete genome 100% 5e-38 100% AY421767.1

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>CVA16_VP2_MSD-AA129-156

EYVLGTIAGGDGNENSHPPYVTTQPGQV

MSD.Genetic Information GAGTACGTGCTGGGCACCATTGCCGGGGGCGATGGTAACGAGAACTCACATCCCCCGTACGTCACCACCCAGCCAGGACAGGTG

Human coxsackievirus A16 strain FY18 from China, complete genome 100% 4e-38 100% EU812514.1

coxsackievirus A16 G-10, complete genome 100% 4e-38 100% U05876.1

Human coxsackievirus A16 isolate CV-A16-genotypeA, complete genome 100% 4e-33 96% JQ746659.1

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